[goodseq]

It's sad. But it's true.

Only for Russians (and Chinese):

good document. thank you.

[diagen]

sequences in Torrent prep

Look here.

I am searching for the sequences used in library preps in Torrent, including immobilized oligos. Could anybody help?

Is it realistic to establish a Torrent library prep on alternative (cheap) reagents? It is a common practice for Illumina DNA and RNA seq.

[weaselfer]

The chips are available but the protocol will not be available until February.

[DICER]

They are on the AB Life Tech pages...

https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=608847

part numbers 4469497 (8 pack) and 4466617 (4 pack)

same pricing as the 316 chips

[aleferna]

How come they are not selling them on their web page? Is it available yet or is it only for early access?

[Geneus]

Has anyone seen any public/customer data on the 318 chip?

http://www.genomeweb.com/sequencing/...er-reports-pag

[singaporeseq]

Has anyone seen any public/customer data on the 318 chip?

[aleferna]

Jejejej thats from today, ok so they are back in the game.

[IonTorrent]

Are they selling the 318, I think we're about to buy the MiSeq, but we can't keep holding on the purchase any longer... any updates on this?

Hi there! The 318 Chip is available:

http://www.prnewswire.com/news-releases/ion-torrent-delivers-on-unparalleled-speed-simplicity-and-scalability-enabled-by-semiconductor-technology-136921308.html

[scbaker]

Looks like they just announced the commercial availability of the 318 chip (more or less on time): http://www.prnewswire.com/news-relea...136921308.html

For me, the surprising statement from this press release was "In the second half of 2012 we expect to increase read lengths on the Ion Torrent PGM up to 400 bases."

Rothberg was talking about 400b reads by the end of 2011 back in June. Has progress really slowed down that much?

[aleferna]

Are they selling the 318, I think we're about to buy the MiSeq, but we can't keep holding on the purchase any longer... any updates on this?

[bioits]

It is in early access (Developer Access section).

[krobison]

I was told today by an Ion Torrent service provider that it is commercially available (though they aren't yet offering it), but I don't see it in the store. Perhaps there is an expanded early access program?

[scbaker]

Thanks for the picture! With smaller wells are we also expecting to see a drop in signal? Wondering what the delay for the 318 is...was originally supposed to be released in Mid 2011...

I don't think the 318 is 'late' quite yet. The 316 was promised (and delivered) mid 2011. 318 was promised 6 months after that, which is right about now. I'm guessing we'll see it by AGBT.

[singaporeseq]

I see, just closer together now...more crosstalk with closer wells then?