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  • austinso
    replied
    Originally posted by IonTorrent View Post
    Avalanche is not Wildfire.
    That's what I heard from the guy who worked on the development of wildfire (he had a poster at ASHG).

    I've heard the following:
    1. no ISPs
    2. isothermal
    3. amplicons directly attached to surface

    FWIW. Interesting to say the least...

    Leave a comment:


  • IonTorrent
    replied
    Avalanche is not Wildfire.

    Leave a comment:


  • thomasblomquist
    replied
    As I suspected.

    Leave a comment:


  • scbaker
    replied
    This appears to be from an Ion Torrent workshop in Russia and my guess is the former (describing both Wildfire and PGM, but not the two working together).

    Leave a comment:


  • thomasblomquist
    replied
    Originally posted by DICER View Post
    Well they are going to let the cat out of the bag ....9th October 2012
    "Mutation Detection and Exome Sequencing Using Wildfire and Ion PGM® Technology" Gijs van Haaften PhD, Netherlands Cancer Institute
    Where did you find this, and is there a description of this presentation? They could be using both Wildfire and Ion Torrent PGM Technology, or Wildfire with Ion PGM technology, based on the wording of the title.

    Leave a comment:


  • DICER
    replied
    Originally posted by nickloman View Post
    Avalanche isn't out now, it's only mentioned as an enabling technology for the PIII chip which is supposed to be 18 months away.
    Well they are going to let the cat out of the bag ....9th October 2012
    "Mutation Detection and Exome Sequencing Using Wildfire and Ion PGM® Technology" Gijs van Haaften PhD, Netherlands Cancer Institute

    Leave a comment:


  • nickloman
    replied
    Originally posted by ymc View Post
    Avalanche seems exciting. Plus, it is out now. Hope to see some user reports soon.
    Avalanche isn't out now, it's only mentioned as an enabling technology for the PIII chip which is supposed to be 18 months away.

    Leave a comment:


  • nickloman
    replied
    Originally posted by steinmann View Post
    400 bp have been announced for 2012 and they already realease one dataset that looks pretty impressive. No word of when the kit is going to be available exactly.
    They announced 400 bp for PGM, not Proton and it's supposed to be by the end of the year. 300 bp for PGM is available.

    Leave a comment:


  • nickloman
    replied
    Originally posted by steinmann View Post
    The press release said it will work for all Ion platforms. Guess that implies beads.
    They said the Ion Chef would work for all Ion platforms, not Avalanche. Different things.

    Leave a comment:


  • ymc
    replied
    Avalanche seems exciting. Plus, it is out now. Hope to see some user reports soon.

    Leave a comment:


  • steinmann
    replied
    Originally posted by flxlex View Post
    I am pretty sure I was told the Proton would launch with 400 bp reads. Can anyone confirm? I'm disappointed they don't go beyond 100 and 200 bp...
    400 bp have been announced for 2012 and they already realease one dataset that looks pretty impressive. No word of when the kit is going to be available exactly.

    Leave a comment:


  • steinmann
    replied
    Originally posted by MrGuy View Post
    As for Avalanche, I wouldn't be surprised if it is Wildfire on Ion. I'd be interested to know if it is still bead-based, however.
    The press release said it will work for all Ion platforms. Guess that implies beads.

    Leave a comment:


  • MrGuy
    replied
    Originally posted by flxlex View Post
    I am pretty sure I was told the Proton would launch with 400 bp reads. Can anyone confirm? I'm disappointed they don't go beyond 100 and 200 bp...
    You are probably linking two separate things which have similar launch dates. Proton is "second half" and 400 bp is "before year end". Personally, I'd rather have a machine with a 10, 100, 1000 G spec on 100 or 200 bp so it'll pump out more data with better/longer chemistries.

    If you are specifically looking for 100% 454 replacement, then I understand where you are coming from.

    Leave a comment:


  • nickloman
    replied
    I've written up some notes and thoughts on the announcement here:



    Lex: When it was announced at AGBT we did the maths on sensors vs throughput and speculated they were targeting 200-250 bases. Most of the marketing literature has since said 200. Not sure I ever heard 400 bases mentioned as a target for Proton.

    Leave a comment:


  • flxlex
    replied
    I am pretty sure I was told the Proton would launch with 400 bp reads. Can anyone confirm? I'm disappointed they don't go beyond 100 and 200 bp...

    Leave a comment:

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