Hi Buzz0r,
did you use the Ion AmpliSeq HiFi Taq mix for the PCR of the panel or another enzyme. I was trying to circumvent buying the Ampliseq mix and I tried to amplify the panel with kappa mix and I didn't observe any products even after more cycles.
Thanks and all the best,
Marlon
did you use the Ion AmpliSeq HiFi Taq mix for the PCR of the panel or another enzyme. I was trying to circumvent buying the Ampliseq mix and I tried to amplify the panel with kappa mix and I didn't observe any products even after more cycles.
Thanks and all the best,
Marlon
Comment