Originally posted by korostin
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1) original AmpliSeq primers: containing target-specific sequences with uracil nucleobases but not containing universal sequences
2) KAPA2G FAST Multiplex Kits: uracil-tolerant polymerase and regular dNTPs (A, C, G and T)
3) genomic DNA
The uracil DNA glycosylase and endonuclease IV treatment for the amplicons followed by the AMPure XP cleaning obtained blunt-ended uracil-less fragments being ready for Illumina library construction.
I hope that answers your question.
Hiro.
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