Greetings all,
I will be doing some single cell transcriptomics soon. I am going to extract RNA from small numbers of bacterial cells (<10) and then circularize (after rRNA depletion) and subject this to MDA using phi29. The resulting cDNA should be able to be shunted into the MuSeek Library Prep kit workflow right? I mean, cDNA can be treated just like sheered gDNA can't it? I can't think of a reason I wouldn't get good reads doing it this way.
I would appreciate any insight you all might have!
Thanks,
Brandon @ASU
I will be doing some single cell transcriptomics soon. I am going to extract RNA from small numbers of bacterial cells (<10) and then circularize (after rRNA depletion) and subject this to MDA using phi29. The resulting cDNA should be able to be shunted into the MuSeek Library Prep kit workflow right? I mean, cDNA can be treated just like sheered gDNA can't it? I can't think of a reason I wouldn't get good reads doing it this way.
I would appreciate any insight you all might have!
Thanks,
Brandon @ASU
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