Tweet
We have an Ion Proton that I will like to carry out fungal ITS amplicon sequencing on. I intend to use ITS1f and ITS 2 primers. However, while I have successfully carried out WGS on the Proton, this is the first time I will be doing amplicon seq. I do know I need to put in barcodes (as I have about 8 samples) and adapter. Would some one have a work flow I can modify or where I can get the necessary information? Is it possible to have custom made oligos with the adapter and barcodes? If so, how do you select your barcodes, their location on the oligos e.t.c?
-
-
Ion has a protocol for amplicon sequencing on their Community site. Look for Fusion Library Protocol. -
Thanks, have now used the one found here http://ioncommunity.lifetechnologies.com/docs/DOC-3322Originally posted by snetmcom View PostComment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment