Hi all ,
I'm working in result from the sequencing of AcroMetrix Oncology Hotspot on PGM iontorrent to validate our pipeline. In the list of the mutation i have a strange case :
As you can see there 50 reads with the mutation in foward position but only 2 reads on the reverse position. I use a strand bias in my pipeline that is why i did not find the mutation in my results. But finally i do not understand why there is a difference between foward and reverse reads.
Do you have any ideas ? It-is an library bias ?
Kind regard
Tristan
I'm working in result from the sequencing of AcroMetrix Oncology Hotspot on PGM iontorrent to validate our pipeline. In the list of the mutation i have a strange case :
As you can see there 50 reads with the mutation in foward position but only 2 reads on the reverse position. I use a strand bias in my pipeline that is why i did not find the mutation in my results. But finally i do not understand why there is a difference between foward and reverse reads.
Do you have any ideas ? It-is an library bias ?
Kind regard
Tristan