Hello everyone
I'm starting to work with the Ion torrent sequencer. I want to sequence fragments that I will amplify using primers forward and reverse.
My question is how do I add the Ion torrent A and P1 adapters to my amplified fragments. I already found the sequences of these adapters in what is published by Ion torrent.
¿Is it necessary to add some recognition sequence to that said adapters can be pasted into a second PCR?
I'm starting to work with the Ion torrent sequencer. I want to sequence fragments that I will amplify using primers forward and reverse.
My question is how do I add the Ion torrent A and P1 adapters to my amplified fragments. I already found the sequences of these adapters in what is published by Ion torrent.
¿Is it necessary to add some recognition sequence to that said adapters can be pasted into a second PCR?
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