I've been told that with the Ion Fragment Kit, the maximum input fragment size should be 150 bp so that with adapters the fragment is no longer than 210 bp.
Can anyone with access to Ion's deepest, darkest secret documents (known elsewhere as "package inserts") confirm this? The application note on Amplicon Sequencing mentions nothing on the subject.
Anyone know if the constraint is imposed in the library prep or the emPCR template prep? What imposes it? Extension times? Nucleotide concentrations? emPCR micelle sizes?
One might guess this is something Ion will need to address in order to hit 454-like read lengths
Can anyone with access to Ion's deepest, darkest secret documents (known elsewhere as "package inserts") confirm this? The application note on Amplicon Sequencing mentions nothing on the subject.
Anyone know if the constraint is imposed in the library prep or the emPCR template prep? What imposes it? Extension times? Nucleotide concentrations? emPCR micelle sizes?
One might guess this is something Ion will need to address in order to hit 454-like read lengths
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