I got (illumina next seq 500) 2x150 bp paired end sequenced mouse transcriptome data.
The vendor gave me a QC report of both Raw and processed data.
In RAW data the max. and min. read length is 151 bp(both) whereas in peocessed data (processed by vendor through NGS QC tool kit) the min and max. read length is 50 and 151 bp respectively.How ? my confusion is that raw data should have all the possible read lengths from 50 to 151 bp ?
Second, i did Fast QC of both, RAW and processed data. Against sequence length distribution window of FastQC,I found normal result with RAW data whereas warning was issued with processed data.
Should i go for down steam processing with RAW or processed data ?
The vendor gave me a QC report of both Raw and processed data.
In RAW data the max. and min. read length is 151 bp(both) whereas in peocessed data (processed by vendor through NGS QC tool kit) the min and max. read length is 50 and 151 bp respectively.How ? my confusion is that raw data should have all the possible read lengths from 50 to 151 bp ?
Second, i did Fast QC of both, RAW and processed data. Against sequence length distribution window of FastQC,I found normal result with RAW data whereas warning was issued with processed data.
Should i go for down steam processing with RAW or processed data ?
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