We are planning to sequence a denovo reference genome of a micro Eukaryote, but cannot extract enough DNA from a single individual for PacBio or Nextera Illumina protocols. We have extracted DNA from 68 individuals of a single population and get 3.56ng total (100ul of 35.6pg/ul). We plan to pool these individuals for a "composite" genome. Our concerns include: 1) are they all the same species/lineage? 2) how useful is a ref genome generated from a composite of individuals? and 3) what will the assembly challenges be with the likely high rate of variation in reads?
We have not seen many publications on this topic. Is anyone aware of publications on such a project or have any thoughts on this?
thanks,
We have not seen many publications on this topic. Is anyone aware of publications on such a project or have any thoughts on this?
thanks,
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