question

[CAROLINE KOSGEI]

Hi everyone, glad to join the group. I made a mistake while preparing libraries for nanopore sequencing at the tail end just before loading to the flow cell for sequencing using the nanopore. Instead of adding sequencing buffer and library beads to the DNA, I instead added AMPure XP beads to the Library beads instead of the buffer. Is it still possible to recover the DNA library and proceed to do the nanopore sequencing. Kindly assist. Thank you

[GenomicSeq]

CAROLINE KOSGEI I think that depends on what volume or ratio you added the beads. If it's a reasonable amount, I think you could just do a bead cleanup and then check to make sure you still have DNA before proceeding. You could always add some buffer to make it the right ratio to save your libraries. My assumption is you will likely lose a bit of your libraries, but with a proper clean up it might not be much at all.

Here's the ratio guide to try and figure out the volume you need to do a proper cleanup: https://www.beckman.com/reagents/gen...pcr/bead-ratio

Best of luck and let me know if you have more questions on this.

[CAROLINE KOSGEI]

Thank you @GenomisSeq Member for the answer. I will use the ratio guide to figure out the volume i will need to do proper clean up. I appreciate your response.