The Tris-HCl is used for the elution from Ampure beads. As far as I know the higher pH facilitates the resolubilization of high molecular weight DNA.
You can definitely use pH8, but you might loose more DNA from the long fraction because it does not come off the beads.
You can definitely use pH8, but you might loose more DNA from the long fraction because it does not come off the beads.
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