Hi all,
I recently constructed a 10k library and sequenced on RS II platform with P4 C2 chemistry, and got an abnormal result of ZMW loading for productivity with P0 74.22%, P1 13.48%, P2 12.3% and mean read length 1,324 bp, mean read quality 0.81.
I refered to the help of smrt portal, which says
"Productivity 0 (%): Percentage of ZMWs that are empty, with no polymerase.
Productivity 1 (%): Percentage of ZMWs that are productive and sequencing.
Productivity 2 (%): Percentage of ZMWs that are not P0 (empty) or P1 (productive). This may occur for a variety of reasons and the sequence data is not usable."
But I still don't know the cause of the abnormal ZMW loading result.
Does P0 means that ZMW has no polymerase loading or no template loading or contaminants deactivate the polymerase?
Anther question is exactly what reasons for P2 occurring ?
Could someone help to explain ? Thx very much.
I recently constructed a 10k library and sequenced on RS II platform with P4 C2 chemistry, and got an abnormal result of ZMW loading for productivity with P0 74.22%, P1 13.48%, P2 12.3% and mean read length 1,324 bp, mean read quality 0.81.
I refered to the help of smrt portal, which says
"Productivity 0 (%): Percentage of ZMWs that are empty, with no polymerase.
Productivity 1 (%): Percentage of ZMWs that are productive and sequencing.
Productivity 2 (%): Percentage of ZMWs that are not P0 (empty) or P1 (productive). This may occur for a variety of reasons and the sequence data is not usable."
But I still don't know the cause of the abnormal ZMW loading result.
Does P0 means that ZMW has no polymerase loading or no template loading or contaminants deactivate the polymerase?
Anther question is exactly what reasons for P2 occurring ?
Could someone help to explain ? Thx very much.
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