Hi everyone,
I have a small problem at the moment.
From our collaborators I got
- 1 assembled genome
- the related PacBio subreads
I did NOT get the smrtcell data (I could ask, but well...not if I can get around it in an easy way).
For my genome submission, I now need to calculate the coverage of my genome. If I map the subreads to the genome, this will not give an accurate result. Therefore I'd like to create the CCS from the subreads.
I'd assume that the protocol "RS_subreads.1" in smrtportal would maybe do something like this. But I cannot even test that, because I cannnot import the subreads, because I don't have the related smrtcell data.
Does anyone have maybe any idea how I could solve this without handling the smrtcell data?
I have a small problem at the moment.
From our collaborators I got
- 1 assembled genome
- the related PacBio subreads
I did NOT get the smrtcell data (I could ask, but well...not if I can get around it in an easy way).
For my genome submission, I now need to calculate the coverage of my genome. If I map the subreads to the genome, this will not give an accurate result. Therefore I'd like to create the CCS from the subreads.
I'd assume that the protocol "RS_subreads.1" in smrtportal would maybe do something like this. But I cannot even test that, because I cannnot import the subreads, because I don't have the related smrtcell data.
Does anyone have maybe any idea how I could solve this without handling the smrtcell data?
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