[spore]

Does anyone have any experience with the new Sequel chemistry on their own system?

[gconcepcion]

Hi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
@DNATECH: Very helpful info!

What issue are you having with samtools?

the new SEQUEL *.bam files are 100% compatible with samtools, we routinely use it internally and it's included as part of SMRTLink

[Gorgon_VZ]

Hi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
@DNATECH: Very helpful info!

[sklages]

Have a look at https://downloads.pacbcloud.com/publ...opsisDemoData/

[DNATECH]

Hi All,
we have only run E.coli libraries with the new chemistry so far.

http://dnatech.genomecenter.ucdavis.edu/2016/11/10/new-service-long-read-sequencing-on-the-pacbio-sequel/

[Gorgon_VZ]

Hi all, has anyone tested the performance of the novel chemistry yet? Any experiences regarding loading efficiency, readoutput, dataquality and so on? I would really, appreciate any kind of information from all you sequel users out there!

Thanks!

[snetmcom]

seems like there is a surprising amount of misinformation. Thanks for clarifying.

[nucacidhunter]

I wonder if this chemistry release is the last one for this year or there will be another chemistry or SMRT cell update before next year.

[mbadgett]

There has never been an NDA for Sequel or its data. Also the updated chemistry is available for order and will start shipping to customers this week.

[GenoMax]

Check out today's blog: http://http://www.pacb.com/blog/sequ...nome-assembly/

Thanks. Does this mean the NDA (if there was one in place) is now lifted and others can start releasing their data?

[GenoMax]

If I am correct the Sequel data in the blog are not from current chemistry.

That data will be available next year

[nucacidhunter]

If I am correct the Sequel data in the blog are not from current chemistry.

[mbadgett]

It is currently in early access testing and released broadly thereafter, before the year end chemistry.

[spore]

Is this using the August chemistry update or is it using the planned EOY chemistry update?

[mbadgett]

So the burning question on everyone's mind. When do we get to see real data from Sequel in wild?

Check out today's blog: http://http://www.pacb.com/blog/sequ...nome-assembly/