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Originally posted by Gorgon_VZ View PostHi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
@DNATECH: Very helpful info!
the new SEQUEL *.bam files are 100% compatible with samtools, we routinely use it internally and it's included as part of SMRTLink
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Hi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
@DNATECH: Very helpful info!
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Hi All,
we have only run E.coli libraries with the new chemistry so far.
Last edited by DNATECH; 11-11-2016, 12:15 PM.
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Hi all, has anyone tested the performance of the novel chemistry yet? Any experiences regarding loading efficiency, readoutput, dataquality and so on? I would really, appreciate any kind of information from all you sequel users out there!
Thanks!
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seems like there is a surprising amount of misinformation. Thanks for clarifying.
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I wonder if this chemistry release is the last one for this year or there will be another chemistry or SMRT cell update before next year.
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There has never been an NDA for Sequel or its data. Also the updated chemistry is available for order and will start shipping to customers this week.
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Originally posted by mbadgett View PostCheck out today's blog: http://http://www.pacb.com/blog/sequ...nome-assembly/
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If I am correct the Sequel data in the blog are not from current chemistry.
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It is currently in early access testing and released broadly thereafter, before the year end chemistry.
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Is this using the August chemistry update or is it using the planned EOY chemistry update?
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Originally posted by GenoMax View PostSo the burning question on everyone's mind. When do we get to see real data from Sequel in wild?
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