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Dear all,
Anyone has such experience? How to conquer the issue of low input DNA?
Thanks,
Wei
Anyone has such experience? How to conquer the issue of low input DNA?
Thanks,
Wei
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Make sure to have Illumina data from the same DNA prep for efficient error-correction
Two points:
1. To get more DNA try making the sample prep more efficient/use more starting material/
2. To allow efficient error-correction of the pacbio reads also prep PCR-free (optional: + Nextera matepair) libraries from the same lot of DNA as used for creating the PAC-Bio libraries, and sequence them at 30X coverage compared to the volume of the raw pacbio data you are getting.
First do self-errorcorection of the pacbio subreads from the same template,
than error correct the filtered pacbio subreads using the Illumina data before the assembly.
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