Set target P1 value at >50%, with the lastest Sequel reagents and SMRT Link v5.1

[weigrc]

Hello all,

Regarding P1 value from Sequel runs, we had been told that at <40% is good while P0 and P2 share the rest evenly.

But refer to the attached, the technical note now is saying to set up P1 value at >50% for Sequel runs. What is your experience with the new Sequel binding kit as V2.1 with SMRT Link v5.1? More usable reads with the same polymerase read length?


Thank you for sharing information in advance.

Wei