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  • Low input fragment library service providers?

    It's not hard to find vendors out there who will take a few micrograms of DNA and generate beautiful Illumina paired end data, even from my 70% G+C samples.

    Any vendors out there offering fragment library construction and sequencing from 100s, 10s or even single-digit nanograms of input material?

  • #2
    If you want to generate complex libraries from these ammounts of starting material I can recommend Illuminas Nextera kit. Dont know about service providers using these kits. Am actually looking for a service provider to sequence Nextera libraries I prepared myself.

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    • #3
      I don't like Nextera. The transposase shearing is not consistent in our hands.

      We routinely make libraries from 1 ng of DNA and a few hundred picograms of RNA. We are too busy to take samples from outside our area though.

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