Is US$9600 cheap for one flow cell 2x100 rapid mode on HiSeq 2500

[GenoMax]

Try the Duke University sequencing estimator here: https://dugsim.net/ No account required.

For one flow cell no library (international institution) estimate seems to e ~$4500 for a rapid 2 x 100 PE flowcell.

[gringer]

Thanks for your reply. I am aware of genohub. But I want to know if my quote is way off or not before I try their service.

You don't need to pay to use genohub. You may need a login, but you can get quotes without any obligation. If you're not prepared to go that far, you're basically asking someone to solve your specific case and not teach you how to solve the more general case -- you might as well just be ignorant to other quotes and pay the $9600.

[ymc]

It seems wrong to do this because I know that Genohub is a frequent answerer, but genohub is a fairly good tool to answer this specific question:

https://genohub.com/shop-by-next-gen-sequencing-project/

You state sample numbers, number of reads (or coverage), preparation, and then it will spit out a list of current service providers and their asking price.

Thanks for your reply. I am aware of genohub. But I want to know if my quote is way off or not before I try their service.

[gringer]

It seems wrong to do this because I know that Genohub is a frequent answerer, but genohub is a fairly good tool to answer this specific question:

https://genohub.com/shop-by-next-gen-sequencing-project/

You state sample numbers, number of reads (or coverage), preparation, and then it will spit out a list of current service providers and their asking price.

[ymc]

I got a quote like this just for sequencing not including library prep or target capture. That's about 50-60Gb data according to the spec.

Is this reasonable? Can I get a quote significantly cheaper than that?

Thanks