New! Encore Complete RNA-Seq Library Systems from NuGEN Technologies

[NuGEN]

The Encore Complete RNA-Seq Library Systems offer a number of advantages for researchers engaged in RNA-Seq studies:

An end-to-end solution for strand-specific RNA-Seq – All required components for low input preparation of strand-specific RNA-Seq libraries from moderate amounts of total RNA without the need for rRNA reduction or poly(A) selection
Superior strand retention – Using a range of sources for total RNA researchers can expect to achieve >95% read mapping in the sense orientation for accurate and complete understanding of transcript profiling results
Affordable and scalable kit configurations – The barcoding capability provided with Encore Complete RNA-Seq Multiplex Systems I and IB improve sample throughput and reduce sequencing costs as your projects grow

http://www.nugeninc.com/nugen/index.cfm/products/amplification-systems/encore-complete-rna-seq-library-systems/

Best,
Steve

[seq2no]

Hi Steve,

We recently sequenced Nugen ENCORE complete RNA seq library samples on our HiSeq 2000. The q30 scores were not great (between 70-80% - we normally see q30s in the 90s). The phasing was high on some of the lanes and the prephasing was high on other lanes. We also happened to run 3 Illumina prepped samples that had normal q30s and normal phasing/prephasing. Have you heard of issues like this before? Do you know why the NuGen prepped samples would behave this way?

Thanks,
Jeanne