Next-generation sequencing (NGS) has revolutionized disease research. It has proven integral in revealing a multitude of novel disease-relevant biomarkers, mechanisms, and pathways, and thus paving the way for the development of new therapeutic approaches. Using NGS to retroactively investigate previously obtained clinical samples is a major part of this process. However, typical clinical sample processing workflows, especially those involving formalin-fixation and paraffin-embedding (FFPE), create samples that are suboptimal for NGS applications.
Download this ebook to learn more about how researchers navigate the challenges that come with working with FFPE samples, from altering nucleic acid extraction workflows to leveraging computational bioinformatics.