The new NEXTflex Cell Free DNA-Seq Kit is optimized to produce libraries from 1 ng of circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA), in two hours or less. This low-input library preparation kit delivers high coverage quality and reduced bias for Illumina sequencing applications. The NEXTflex Cell Free DNA-Seq Kit contains a high fidelity enzyme which exhibits minimal GC bias and produces uniform coverage of difficult to sequence DNA. The patent pending Enhanced Adapter Ligation Technology incorporated into the ligation step offers the highest ligation efficiency available, resulting in greater library diversity and a larger number of unique sequencing reads. Up to 192 unique adapter barcodes are available, facilitating high-throughput multiplexing. Protocols for automating library prep using the NEXTflex Cell Free DNA-Seq Kit are also available, for Bioo Scientific's SEQBOT™ NGS Library Prep Automation Platform, the Beckman Biomek® FX and Biomek® FXP Laboratory Automation Workstations, and the Sciclone NGS, Sciclone NGSx and Sciclone G3 platforms.
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Last edited by Bioo Scientific; 10-23-2014, 09:00 AM. -
The NEXTflex Cell Free DNA-Seq Kit is designed for making NGS libraries from low-input cfDNA or ctDNA samples. Visit our website for data demonstrating the use of this kit to produce high-quality informative libraries from cell-free DNA extracted from plasma. -
Do you have any data that demonstrates superiority of your ligation technology in comparison to other similar products? And can you explain to your potential customers why your library protocols are not freely avaible online like other vendors?Last edited by nucacidhunter; 10-15-2014, 11:55 AM.Comment
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I have question. Is nextflex compatible with Ion proton sequencer?Do You provide adapters and other components separetly?Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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