The NEXTflex™ mtDNA-Seq Kit includes optimized reagents for the isolation of mitochondrial (mtDNA) and construction of Illumina-compatible paired-end or single read libraries from genomic DNA. The NEXTflex mtDNA-Seq Kit enables selective enrichment of mitochondrial DNA from total genomic DNA samples by selectively digesting linear nuclear DNA (nDNA) while leaving circular mtDNA intact; enriching the mitochondrial genome 100 – 350 times. After enrichment libraries prepared using the NEXTflex mtDNA-Seq Kit produce a greater number of unique reads reducing the number of sequencing reads required per sample.
A whitepaper describing the mtDNA isolation and library prep technology can read here.
A whitepaper describing the mtDNA isolation and library prep technology can read here.