Bioo Scientific recently launched the NEXTflex-HT™ Barcodes, single-index barcodes added during the ligation step of library prep, which offer deep multiplexing for both paired-end and single-read Illumina sequencing. Each barcode contains a unique 12 nt index sequence with a Hamming Distance of five or greater, permitting dual error correction to enable proper differentiation between samples. Additionally, each pair of consecutive barcodes is suitable for low level multiplexing. Currently 384 barcodes are available; however, > 2,000 are being developed.
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM