Hi everyone. With the ScriptSeq mRNA-Seq Library Preparation Kit, would you recommend the MinElute or gel electrophoresis for library purification?
Has anyone had experience of primer dimers after the PCR of 10 cycles? Cutting the band via size selection from a gel would perhaps then be the better choice. However, we do realise that by using the MinElute we can avoid exposing our sample to UV and Ethidium bromide and so we will probably obtain a much purer sample from MinElute as well as it being much a much easier protocol. Any comments on this would be greatly welcomed!
Also, will our library still include the tRNA and small RNAs? Our bioanalyser run of the rRNA depleted RNA has shown that we have quite a lot of tRNA and we were wondering if they will be in the library then we can avoid them by size selection on the gel?
Many thanks for your help!
Has anyone had experience of primer dimers after the PCR of 10 cycles? Cutting the band via size selection from a gel would perhaps then be the better choice. However, we do realise that by using the MinElute we can avoid exposing our sample to UV and Ethidium bromide and so we will probably obtain a much purer sample from MinElute as well as it being much a much easier protocol. Any comments on this would be greatly welcomed!
Also, will our library still include the tRNA and small RNAs? Our bioanalyser run of the rRNA depleted RNA has shown that we have quite a lot of tRNA and we were wondering if they will be in the library then we can avoid them by size selection on the gel?
Many thanks for your help!
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