Hi folks,
I have extracted DNA from fungi for whole genome sequencing (Truseq PCR free with 350 bp insert and HiSeq 2500 PE) using a kit....
40 results in 0.0024 seconds.
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Please help! DNA samples for whole genome sequencing
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What is RNA bait?
Can anyone explain how to design RNA bait for whole genome sequencing? -
CNV detection tools for non-human genomes
Hi all,
I'm very new to the world of genomics and CNVs so forgive me if my question is a bit basic.
I will be looking into detecting... -
Base call pairs drifting apart over the course of a MiSeq run
Hello everyone,
We've been having problems with our base calls, especially A and T, diverging over the course of a run (see attached screen... -
Include heterochromatin in genome size estimates?
I am planning on sequencing several entire drosophila (fruit fly) genomes for an upcoming project. The published length of the drosophila genome is ~150... -
Newbie bwa question (HiSeq data)Hi, Sorry for the newbie question! I am trying to c
Hi,
Sorry for the newbie question!
I am trying to convert output from HiSeq whole genome sequence to bam format that will... -
WGS batch effects
Hi All,
I'm relatively new to whole genome sequencing, and have a background in 16S based typing of bacteria using NGS. I am collecting... -
Epignome Methyl-Seq
Hello Everyone,
Anyone has experience with Epicentre's Epignome Methyl-Seq kit? I recently started using it and my yields have been too... -
Illumina TruSeq PCR-Free - Low Final Concentration WGS Rat DNA Libraries
We are trying to run whole genome sequencing on ~40 rats for a project. We performed the preps following the TruSeq DNA PCR-free LT protocol for the 550bp... -
High Error Rate After Repeat In Reads
I have obtained whole genome sequencing data for human patients. I notice that after a repeat region, the rest of the read is typically soft-clipped.... -
Nextera Kit -- DNA fragments too small
Hello --
I have been working with the Nextera kit for Illumina to create DNA libraries for genome sequencing. I initially used the recommended... -
how to analyze whole genome sequencing data (new genome assembling)?
This is a project to sequence the whole genome of a species (which has not been sequenced yet), assemble the reads, and use the annotations (from related... -
Bioinformatic postdoctoral position at MSKCC, New York
We currently have a postdoctoral position for a highly motivated candidate with a background in bioinformatics interested in investigating inter- and...Last edited by Jorge Reis-Filho; 03-14-2013, 06:28 AM. -
sam-dump -u from sra file fails to output unaligned reads
Hi,
I downloaded an sra file of whole exome seq.
When trying to convert it to a sam file I use the -u flag to get the unaligned reads. However,... -
BSSeq on whole genome - BS befor library prep?
Hi everyone,
My first post, so inform me if I'm not following the rules!
I am planning to perform BSSeq on genome scale on microbial...
