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Hi folks,
I have extracted DNA from fungi for whole genome sequencing (Truseq PCR free with 350 bp insert and HiSeq 2500 PE) using a kit. The extraction went smooth until I realized at the end that I treated my samples with only half of the recommended amount for RNase A (2 ul of 10 mg/ml instead of 2 ul of 20 mg/ml). I tried checking the DNA samples on 1% agarose and following is the gel image with 100-1000 bp ladder.
I am not sure there are RNA bands (very faint though) at around 100-200 bp. Do you think my DNA samples are good enough to go for whole genome sequencing without additional treatments? Or shall I try to treat them with RNase A again? Unfortunately, I run out of fresh samples.
I appreciate all your comments and suggestions.
Best
Vinn
I have extracted DNA from fungi for whole genome sequencing (Truseq PCR free with 350 bp insert and HiSeq 2500 PE) using a kit. The extraction went smooth until I realized at the end that I treated my samples with only half of the recommended amount for RNase A (2 ul of 10 mg/ml instead of 2 ul of 20 mg/ml). I tried checking the DNA samples on 1% agarose and following is the gel image with 100-1000 bp ladder.
I am not sure there are RNA bands (very faint though) at around 100-200 bp. Do you think my DNA samples are good enough to go for whole genome sequencing without additional treatments? Or shall I try to treat them with RNase A again? Unfortunately, I run out of fresh samples.
I appreciate all your comments and suggestions.
Best
Vinn
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