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  • Faria
    Junior Member
    • Dec 2014
    • 9
    #1

    Epignome Methyl-Seq

    Hello Everyone,

    Anyone has experience with Epicentre's Epignome Methyl-Seq kit? I recently started using it and my yields have been too low for sequencing. If you have used the kit... I need help optimizing the protocol before I run out of the kit What is your input concentration, number of PCR cycles and yields? Any suggestions would be greatly appreciated.

    Thanks in advance
    Tags: epigenetics, whole genome sequencing
  • nucacidhunter
    Jafar Jabbari
    • Jan 2013
    • 1250
    #2
    I have used the kit with 50 ng input following exact kit protocol and libraries yielded around 2 ng/ul. Sequencing result also was good with around 75% mapping to reference.

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