Hello,
I am having an issue with library pools rapidly degrading after pooling and size selection.
I am prepping low biomass...
143 results in 0.0031 seconds.
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Library degrading after prep with Ovation Ultralow V2
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16S sequencing from total RNA?
I'm working with a researcher that wants to identify the bacterial communities present in human dental pulp tissue, blood, and saliva from isolated RNA.... -
FastQC results for 16s reads
Hi
I have some FastQC results I have generated from Illumina 16s sequences. I have attached an example of the results for one of the demultiplexed... -
metagenomics tool
Gaia 2.0 is now online:
- valid for any type of analysis (16S/18S, ITS, WGS, metatranscriptomics)
- valid for any type of sample... -
Simple statistics help
Hello there,
I am currently working on a metagenomics project on the oral microbiome. I am new to metagenomics and not the best at statistics,... -
V4-V5 Region Using 2x250 - Enough Overlap?
Hi All,
We've been looking at the V4 region (515/806) in environmental samples for some time successfully. We'd like to move to the V4-V5... -
SsarahP started a topic Crowdsourcing the Advancement of Microbiome Research: Mosaic & precisionFDA Challengein MetagenomicsCrowdsourcing the Advancement of Microbiome Research: Mosaic & precisionFDA Challenge
Two microbiome challenges launched this week with a common goal: evaluate and advance the ability of computational methods to accurately detect known... -
MetaSUB Forensics Challenge @CAMDA ISMB Chicago
The MetaSUB International Consortium (metasub.org) is building a longitudinal metagenomic map of mass-transit systems and other public spaces across the... -
MetaSUB Forensics Challenge @CAMDA ISMB Chicago
The MetaSUB International Consortium (metasub.org) is building a longitudinal metagenomic map of mass-transit systems and other public spaces across the... -
Training in Metagenomics Data Analysis @NIH, USA, Nov 14 - 17, 2017
Hands-on Training in Metagenomics Data Analysis
Nov 14 - 17, 2017
Where?
National Institutes of Health... -
Pre-doctoral research fellow in bioinformatics - metagenomics
We need an outstanding candidate excited about novel approaches for the metagenomics data analysis
Our group, together with MetaSUB (w... -
raw illumina reads or assembled contigs for Taxonomy
Hello!
I am having whole genome sequenced metagenomic data that is assembled using meta-velevt. I want to check taxonomic classification... -
IDBA 'std::logic_error'
Hi, I am trying to merge paired-end illumina reads (read length: 101) .
I am using the command:
fq2fa --merge --filter sample_1.fq... -
Advice on assembling very large metagenomic dataset?
I need to assemble a large metagenomics dataset from Illumina NextSeq reads. My read depth is approximately 20 million reads per sample (28 samples)...Last edited by jol.espinoz; 03-01-2017, 12:16 PM. -
Fastest way to add Ns to variable length sequences to get uniform length
I have a fasta file with a thousand sequences with a distribution of lengths between 100 and 150bp. I would like to add Ns to all sequences whose length...
