Dear all,
I would like to create two complete bacterial genomes from two recently described bacteria. I have draft genomes for both. My...
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Creating bacterial reference genomes
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Beginner. basic parameter for MIRA using 454 FLX data
Hi.
i am a beginner in the de novo assambly of genome and have a big question. I am sequence the bacteria using 454 FLX and need assembler... -
Sequencing for enzyme discovery - Miseq or 454?
Hi everyone,
I'm very new to the world of next generation sequencing, I've been reading a lot about the different types and their advantages... -
454 data Assembly editing in GAP5 : Join contigs function
Hi,
I am trying to reduce the number of contigs I got after an assembly run using Mira on 454 single end data. For Assembly editing, GAP5... -
de novo assembly with MIRA and 454 single-end reads. Too much contigs
Hi everybody!
I'm new in NGS data processing and also in this great community.
I have two sets of reads 454 single-end from Roche... -
Do I have to install velvet twice to run color and base space?
Hello, I want to install velvet to run sometimes sequence space (454), some times color space (SOLiD), and sometimes both.I see that in the installation... -
Celera Assembler seems to discard HiSeq reads
Ran Celera Assembler on HiSeq and 454 reads separately and got same results. Are there any specific tweaks one should use for HiSeq reads and 454? -
Mitochondrial Genome Assembly
Hi all,
I'm new to the bioinformatics world and am looking for an assembly/alignment program that would work well for the mammalian mitochondrial... -
MIRA assembly with MID barcodes for 454?
MIRA assembly with MID barcodes for 454?
How can you do it?? -
QC of 454 reads
Hi,
I wonder how people analyze the quality of the 454 Titanium reads? What softwares/tools/pipelines exist? I was thinking of converting the SFF... -
Newbler segmentation fault
I'm using Newbler x86_64 version to assemble my 454 reads on a cluster machine. When I try to use this command:
...Code:addRun -lib 3kb_PE project2 GE
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Input files for Roche GSMapper
I am using GSMapper to assemble 454 reads to a reference bovine genome.
I would like to specify a reference genome annotation and so that my variations... -
Using PHRAP to assemble 454 contigs and Sanger reads
Hi all,
I'm a beginner in sequence assembly and have a question about using the assembler, PHRAP.
I'm trying to use PHRAP... -
Runs of Ns in Velvet assembly?
Hi All,
Recently got bacterial genome assembled using Velvet. Reads were generated from 454. The assembly got quite a bit of runs of "Ns"....
