Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties....
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in ArticlesExpert Advice on Automating Your Library Preparations
Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties.... -
Novel Automation - Feedback Welcome
Hello,
I'm looking to speak to NGS users about sample preparation pain points, and automation needs. Leveraging novel fluidic solutions,...
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Hamilton On Deck Thermal Cycler
Does anybody have experience with Hamilton STAR + On Deck Thermal Cycler? How walk-away/automated does this make NGS preps? I'm interested in adding this...
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Over 150 of Automated NGS Methods Available
With more than 150 standardized and tested methods developed for the Sciclone®, Zephyr® and JANUS® G3 NGS liquid handlers, PerkinElmer offers a large...
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Opentrons Automated Pipetting System
Has anyone here used the Opentrons OT-2 for library preparation? https://opentrons.com/ot-2
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Streptavidin Bead Quantitation
Hi Everyone!
I am trying to evaluate how well our new robot handles bead clean-ups with streptavidin beads. I would like to objectively quantitate...
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Automating consensus sequence generation...?
I have a rather straightforward problem. I'm doing a pop. gen. study which has ~1200 individuals. Using our Miseq, I've generated short (~150b) fragments...
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Evaporation During End Repair on Automated Platform
My company is trying to run A-tailing and End repair on an automated liquid handler. During one of the steps, the temperatures get up to 65C and causes...
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Liquid Handling Instruments
Hello everyone,
First time posting here in a long time...good to be back!
My lab currently uses three of the Agilent Bravo...
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Automated script for paired end sequencing
Hello everybody,
I have a folder with paired end sequencing data (divided by the asterisk to make group clearly visible here). I would like...
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Volumetric scaling for library prep
Hi folks,
Looking over some of the early papers showing the equivalence of volumetrically scaled Nextera reactions has me wondering which...
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The Best Robot for NGS Library Prep
Hi everyone,
I wanted to get some insight on a robot for NGS library prep. I am a Lab Mgr. of a NGS core that currently runs 2 MiSeqs....
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System developer and administrator to NGI-Stockholm Core Sequencing Facility
System developer and administrator
We are looking for a system administrator and developer to join our production team to develop and maintain...