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  • Opentrons Automated Pipetting System

    Has anyone here used the Opentrons OT-2 for library preparation? https://opentrons.com/ot-2

  • #2
    "Accuracy Accurate down to 384-well plates"

    This is a new measure of accuracy that I was previously unaware of.

    Their only 'Sequencing Application' in their protocol library is for 16S. There are liquid handling platforms with a much wider array of NGS protocols ready to go than this..

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    • #3
      A neighboring lab has the OT-One. This first version is unusable for just about anything - it can't hold the pipettes properly and the plates have a lot of play. Version two looks very different, though.

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      • #4
        There have recently been a few discussions about them on Reddit; perhaps it might be a way to get in touch with people who use them.

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        • #5
          We recently got an OT2. So far it works for single channel pipetting.

          We have problems with both of our multi-channel pipettes.
          The magnetic module seems to have a design flaw - the magnet is too close to the samples, even when disengaged and at maximum distance (achieved with an self-made adapter). Thus, the beads do not stay in suspension for long - ideally they should stay in suspension for 5 minutes before then engaging the magnet.

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