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SMRT Tools HiFiViral_SARS-CoV-2_M13barcodes?
Hello, I'm a bioinformatics analyst trying to run some coronavirus samples through the HiFiViral SARS-CoV-2 Analysis application, a part of SMRT Tools.... -
Choosing the best hexamer sequences for custom adapters?
I have two sets of adapters that I am using for my library preparations. Both are custom adapters.
Universal Y-shaped Adapter 1:
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script for checking barcode compatibility
Hi everyone,
a colleague of mine wrote a script that checks the compatibility of barcodes used in different sample prep kits for Illumina... -
Quality check with barcordes
Hello guys!
I'm really beginner at bioinformatics.
I work with viral metagenomics and my library is going to be constructed with Nextera... -
processing Fastq files from HiSeq2000 single end and RNAseq analysis
Hello all,
i apologise in advance for asking some basic questions
i have recently started working on RNA-Seq. i have fastq... -
fusion primers for Illumina Miseq
When I did 454 I just amplified my gene using fusion primers that had the gsp, then the barcode then the adaptor primer all in one oligo (barcoded forward... -
Best alternative for Nextera Indices for multiple amplicon resequencing experiment ?
Hi all,
after using the 96 plex double indexing kit (8 X 12 indices) of Illumina for 16s studies, we now apply this for other amplicon... -
Adapter artifacts 16S library prep from low density samples
Hi there!
I'm new to the world of library prep for MiSeq thought I would reach out to the community for some suggestions. We are preparing... -
RNA-Seq, more than 16 barcodes possible?
Hello, i will be sequencing miRNAs, and would like to know if it's possible to obtain barcodes from any 3rd party vendors. I'm aware that the DNA barcodes... -
Multiplexing more than 384 samples on a MiSeq run
Does anyone know of any good methods for multiplexing more than 384 samples on a MiSeq run? The Nextera XT Index Kit allows indexing for a maximum of... -
effective mismatches allowed while demultiplexing
Hi all,
What is the minimum/optimum number of mismatches to be allowed during demultiplexing ?? How do we decide this?
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Lots of "N" in the barcode.
Dear All
I am new to NGS and GAII.
After several test running ,we have a big problem
.
We usually... -
Xsq multiplexing recovery
I used the SOLID 5500xl for my first barcoded run. The run was fine however I noticed a very poor assignment of the barcodes. I traced the problem to... -
Barcodes & biases
Hi there,
Has anyone observed barcode-specific biases in his/her NGS samples?
I am aware of the miRNA-seq papers on that... -
TruSeq Library Pooling - Balanced Indexes?
Hi:
I typically pool 6 TruSeq libraries with P5/P7 adapter pairs (2 4 5 6 7 12) per lane, as per Illumina's recommended pooling for balanced...
