Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • SMRT Tools HiFiViral_SARS-CoV-2_M13barcodes?

    Hello, I'm a bioinformatics analyst trying to run some coronavirus samples through the HiFiViral SARS-CoV-2 Analysis application, a part of SMRT Tools. I need to demultiplex the ccs.bam files I have (see "Preparing Input Data, pg. 44: https://www.pacb.com/wp-content/uplo...uide_v10.2.pdf).

    The guide states to "Provide the proper barcode sequences: HiFiViral_SARS-CoV-2_M13barcodes." Okay, neat, sounds super easy, barely an inconvenience.

    I can't find those anywhere. Not on GitHub, not in the project metadata, not on forums. I emailed PacBio and didn't get a response. Does anyone have a lead on where I need to go/what I need to do to acquire these secret barcodes?

    Thanks

  • #2
    The barcodes are on the pacb.com website



    Click on the "Product Details+" below barcoded M13 primer plate. The barcode sequences are in the Primer Sequences (FASTA) link.

    Cheers,
    John

    Comment


    • #3
      Super, thanks a lot!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        05-06-2024, 07:48 AM
      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. ā€œEpitranscriptomics brings together the concepts of epigenetics and gene expression,ā€ explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 05-14-2024, 07:03 AM
      0 responses
      19 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-10-2024, 06:35 AM
      0 responses
      43 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-09-2024, 02:46 PM
      0 responses
      53 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-07-2024, 06:57 AM
      0 responses
      42 views
      0 likes
      Last Post seqadmin  
      Working...
      X