SMRT Tools HiFiViral_SARS-CoV-2_M13barcodes?

[el239]

Hello, I'm a bioinformatics analyst trying to run some coronavirus samples through the HiFiViral SARS-CoV-2 Analysis application, a part of SMRT Tools. I need to demultiplex the ccs.bam files I have (see "Preparing Input Data, pg. 44: https://www.pacb.com/wp-content/uplo...uide_v10.2.pdf).

The guide states to "Provide the proper barcode sequences: HiFiViral_SARS-CoV-2_M13barcodes." Okay, neat, sounds super easy, barely an inconvenience.

I can't find those anywhere. Not on GitHub, not in the project metadata, not on forums. I emailed PacBio and didn't get a response. Does anyone have a lead on where I need to go/what I need to do to acquire these secret barcodes?

Thanks

[jharting]

The barcodes are on the pacb.com website

https://www.pacb.com/products-and-services/consumables/multiplexing-kits/

Click on the "Product Details+" below barcoded M13 primer plate. The barcode sequences are in the Primer Sequences (FASTA) link.

Cheers,
John

[el239]

Super, thanks a lot!