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SMRT Tools HiFiViral_SARS-CoV-2_M13barcodes?

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  • SMRT Tools HiFiViral_SARS-CoV-2_M13barcodes?

    Hello, I'm a bioinformatics analyst trying to run some coronavirus samples through the HiFiViral SARS-CoV-2 Analysis application, a part of SMRT Tools. I need to demultiplex the ccs.bam files I have (see "Preparing Input Data, pg. 44: https://www.pacb.com/wp-content/uplo...uide_v10.2.pdf).

    The guide states to "Provide the proper barcode sequences: HiFiViral_SARS-CoV-2_M13barcodes." Okay, neat, sounds super easy, barely an inconvenience.

    I can't find those anywhere. Not on GitHub, not in the project metadata, not on forums. I emailed PacBio and didn't get a response. Does anyone have a lead on where I need to go/what I need to do to acquire these secret barcodes?

    Thanks

  • #2
    The barcodes are on the pacb.com website

    https://www.pacb.com/products-and-se...iplexing-kits/

    Click on the "Product Details+" below barcoded M13 primer plate. The barcode sequences are in the Primer Sequences (FASTA) link.

    Cheers,
    John

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    • #3
      Super, thanks a lot!

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