Hi all. I think I've read all the old similar threads, but none of them had a definitive solution and I'm still stuck with this issue. So...
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H3K27ac ChIP - Why do I get mostly large fragments IP'ed shown by Bioanalyzer pre lib
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bioanalyzer "sawtooth" pattern in smartseq2 libraries?
Hi all,
I wonder if anybody has ever come across this sawtooth appearance in smartseq2 libraries? I have no idea what could have caused this,... -
10x genomics scRNA-seq bioanalyzer traces
We are quite new to the single cell RNA-seq world and was wondering if others have seen cDNA bioanalyzer traces like ours (in attachments)
Note... -
Preferred analyzer for library size/library quality
Hello! I am new to the world of NGS and am curious about the preferred instrument for checking library quality. The ones that seem most popular are Agilent... -
bioanalyzeR: New R package for data from the Agilent Bioanalyzer and TapeStation
I wrote an R package for importing electrophoresis data from the Agilent Bioanalyzer and TapeStation and graphing the results with ggplot2 or analyzing... -
bioanalyzer and qubit disagreement
We prepare clinical samples with a formal SOP and do not regularly see irregularities. Today we have a capture pool of samples that quanted at 24.0ng/uL... -
ATAC Library from ffpe
Hi all,
Does anyone has an example of bioanalyzer traces of ATAC libraries prepared from ffpe?
Thank you!
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28s Missing After gEliminator
I have been extracting RNA from heart tissue for almost 10 years. I usually use the Qiagen RNeasy kit with on column DNAse digestion. This month I tried... -
ScriptSeq v2 135base-peak
Hello people,
I tried the ScriptSeq v2 RNA Library Prep Kit on the total RNA extract of a cerebrospinal fluid sample. The RNA amount was... -
ddRAD Post-PCR Bioanalyzer Has Multiple Peaks
Hello,
I have a problem with a post-PCR ddRAD library that was prepared using Peterson (2012). It was prepared along with another index... -
crustacean small RNA library QC
Hi, everyone,
We're trying for the first time to prepare small RNA libraries for our crustacean model, and we're using the NEBNext small... -
NexteraXT Bionalayzer - undertagmentation?
Hi,
I am using the NexteraXT kit to prep mtDNA. I just did a test prep of some samples and had them run on a HS DNA Chip on the Bioanalyzer... -
Bioanalyzer/TapeStation fragment sizes larger than agarose gel
I am preparing libraries for GBS, and although the agarose gel image is as expected (i.e. the bulk of fragments between 200 and 400bp), TapeStation result...Last edited by cpylant; 10-27-2017, 08:56 AM. -
Two cDNA Peaks on Bioanalyzer
I used 200 ng total RNA input and the KAPA HyperPrep Kit to generate cDNA libraries. Usually, our libraries look like Sample 1 in the attached pdf, but... -
Problematic ddRAD library post-size selection bioanalyzer trace
Hi everyone,
I'm preparing a ddRAD-seq plate for Illumina HiSeq 4000 sequencing and I'd like to know if anyone has seen similar Bioanalyzer...
