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  • jbrandim
    Junior Member
    • Sep 2011
    • 5
    #1

    28s Missing After gEliminator

    I have been extracting RNA from heart tissue for almost 10 years. I usually use the Qiagen RNeasy kit with on column DNAse digestion. This month I tried the RNeasy Plus kit that comes with gEliminator tubes (to remove genomic DNA contamination). When I ran a bioanalyzer to confirm quality I noticed that all the samples I extracted with the gEliminator tubes are missing the 28s peak. Has anyone else ever noticed this?

    With gEliminator step
    Click image for larger version Name: 20190116_gElimin.PNG Views: 1 Size: 86.6 KB ID: 309557

    With RNAse free DNAse on column digestion
    Click image for larger version Name: Capture.JPG Views: 1 Size: 85.0 KB ID: 309558
    Tags: 28s, bioanalyzer, qiagen, rna, rneasy
  • jbrandim
    Junior Member
    • Sep 2011
    • 5
    #2
    Update: Talked with Qiagen. The problem seems to be that Qiazol is not compatible with the gEliminator column, because of the acidity.

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