Hi,
I'm currently trying to use the CogentAP pipeline to process some bulk rna seq from murine sorted cells. We used the SMARTer Stranded...
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SPAdes assembler
Hello,
I tried to assemble a mitochondrial genome assembly with SPAdes assembler using pair-end Illumina and Nanopore sequencing data. The size...
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Software Developer Interested in Building Bioinformatics Software
Hello All,
I am a professional developer and software architect with a strong interest in bioinformatics (I studied biology, genetics and computer...Last edited by SoftwareGuy; 09-24-2019, 02:33 AM.
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ISCN Cytogenetic Nomenclature - from genomic coordinates
Hi,
I got a large table with genomic coordinates of CNV found in our cohort:
I would like to add the following data...
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bcftools - retain low quality read position support?
Is it possible to have `bcftools call -c` output a DP4 values that add up to the total DP value. I want to call simple consensus sequences based on a...
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Error while using tool for linkage analysis.
Hey all.. I am currently trying to use LINKDATAGEN and facing a frequent problem mentioned by many users. My sample set is human and I performed exome...
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Methods of locating new proteins' location on genome?
Is there a method of finding out where a unknown protein (doesn't give any good BLAST hits) would be located on human or virus genomes?
...
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miRNA analysis
Hello all,
I am a newbie at bioinformatics and am trying to analyse some miRNA data from the SOLiD sequencer I.e. color space csfasta format....
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Biopython SeqRecord
Hi everyone
I`ve a trouble with adding letter annotations into my sequence.
I have a Seq object and a string with secondary structure...
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Editing genome assemblies with Cytoscape
Hi All,
I have performed a metagenomic assembly from Ion-Proton reads. Now I want to check my assembly using Cytoscape for probable misassembles....
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Clustering of samples based on tetra nucleotide frequency
Hi All,
I have assembled several metagenomic samples of various sequencing depth. The contigs have been annotated with COG. Now I want...
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How to append fasta files
Hi,
I have a series of snp and indels (csv format) for which I know the exact location on the the human genome (chromosome# and nucleotide...