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Hello all,
I am a newbie at bioinformatics and am trying to analyse some miRNA data from the SOLiD sequencer I.e. color space csfasta format. I think I have managed to align these to pre-miRNA and mature sequences to produce SAM files.
My question is what to do next? I need to now count the frequencies of each miRNAs and carry out normalisation so these are relative frequencies from each sample??? How can I achieve this and is there then a nice way to present this data? Or am I barking up the wrong tree and if so what do you advise??
I would be very appreciative of any advice from more experienced bioinformaticians.
I am a newbie at bioinformatics and am trying to analyse some miRNA data from the SOLiD sequencer I.e. color space csfasta format. I think I have managed to align these to pre-miRNA and mature sequences to produce SAM files.
My question is what to do next? I need to now count the frequencies of each miRNAs and carry out normalisation so these are relative frequencies from each sample??? How can I achieve this and is there then a nice way to present this data? Or am I barking up the wrong tree and if so what do you advise??
I would be very appreciative of any advice from more experienced bioinformaticians.
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