Tools for identifying/verifying protein (without accession number)
If I have a series of protein sequences (experimentally obtained) that yield no results from BLAST, are there any other online/software tools that can be used to either identify or verify that these proteins exist?
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If you obtained these sequences experimentally then they definitely exist
Have you tried psi-blast or better yet delta-blast @NCBI? -
They're not annotated in databases such as UniProt/Swiss-Prot so I'm not sure how I can "prove" that they are indeed real and transcribed from genes.
All I have are the protein sequences themselves though, won't I need accession numbers or ID to run them through psi/delta-blast?Comment
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You just need fasta sequence for doing delta- or psi-blast. You can limit your search against swissprot or refseq if you want well annotated proteins.Comment
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