Hi,
I am planning to do ChIRPseq which requires sonication of chromatin down to around a 100-500bp range. The protocol recommends using...
8 results in 0.0031 seconds.
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covaris sonication for ChIRPseq
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Anyone using Bioruptor Pico?
Hi,
I'm wondering whether anyone here has ever succeeded using Bioruptor Pico sonicator for transcription factor ChIP-qPCR.
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Incomplete shearing with Bioruptor?
Hi all!
Shearing gDNA for NGS sample prep. I'm trying to shear my DNA into 500bp fragments using the bioruptor and have two problems: ... -
Position-specific GC bias?
Hi, I've been having an unusual problem with my reads. I've been doing exome sequencing on a HiSeq 2000, but I'm getting only about 40% GC content at... -
Bioruptor (serious) decreasing efficiency overtime
Dear mates,
I've been extensively using the Bioruptor for shearing chromatin for ChIP applications. I came to use different machines of... -
Bioruptor - DNA in TE?
Various protocols for bioruptor shearing suggest that you reconstitute your DNA to your desired concentration in TE (e.g. 1ug DNA in 100ul TE). My DNA... -
Sonicator
Hi all,
We are not quite ready to invest in a Bioruptor and wanted to know what sonicators people are using out there. We've been using a collaborators... -
High-Throughput DNA Shearing
Hello,
Does anybody have any experience or recommendation about DNA shearing tools that can handle multiple samples simultaneously?...Last edited by ABSOLiDuser; 03-09-2009, 07:35 PM.
