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High-Throughput DNA Shearing

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  • High-Throughput DNA Shearing

    Hello,


    Does anybody have any experience or recommendation about DNA shearing tools that can handle multiple samples simultaneously? I know there are some systems out there that can sonicate a whole plate. Has anybody tried them? Also, I have seen several posts on the Bioruptor: does anybody know if it can go down to 150-200bp peak size? How long does it take?

    Thank you so much!
    Last edited by ABSOLiDuser; 03-09-2009, 07:35 PM.

  • #2
    We are working on a way to fragment and add adaptors using a transposon-based method. Our fragments tend to smear in the 150-2000 bp range. How critical is it for you to be in the 150-200 bp range? We can gel purify, but would like to eliminate this step if possible. Which adaptors do you need to add?

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    • #3
      Thanks EPI.
      Last edited by ABSOLiDuser; 03-09-2009, 07:34 PM.

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      • #4
        Piranha

        Hi,

        I believe ABI is working on a 96 well sonicator for SOLiD called a 'Piranha'. I heard something about it when I attended a SOLiD V3 launch seminar. Talk to your rep and see if this is the case.

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