Dear mates,
I've been extensively using the Bioruptor for shearing chromatin for ChIP applications. I came to use different machines of different ages from different labs.
I noticed something really worrying for which I did not find any report yet : I have a very strong feeling that the Bioruptor machines endure a serious decrease of their efficiency overtime (and quite quickly, after 1 year of service), unfortunately leading to a very low reproducibility of the sonication.
A first machine from a nearby institute is ≈3-4 years old and is just not sonicating anything at all ; a second one from another institute is ≈1 year old and requires increased sonication cycles from one experiment to another to achieve a given sonication profile.
I also previously used "conventional" sonicators, which were also showing a decrease of their efficiency and thus required the probe to be regularly (= every 6 months) changed.
With the Bioruptor, there is - to my knowledge - just nothing to be changed. But the machine must somewhat wear-out from the ultrasound waves, as "conventional" sonicators do.
I really interested to know whether your experienced a similar observation with your own machines.
I've been extensively using the Bioruptor for shearing chromatin for ChIP applications. I came to use different machines of different ages from different labs.
I noticed something really worrying for which I did not find any report yet : I have a very strong feeling that the Bioruptor machines endure a serious decrease of their efficiency overtime (and quite quickly, after 1 year of service), unfortunately leading to a very low reproducibility of the sonication.
A first machine from a nearby institute is ≈3-4 years old and is just not sonicating anything at all ; a second one from another institute is ≈1 year old and requires increased sonication cycles from one experiment to another to achieve a given sonication profile.
I also previously used "conventional" sonicators, which were also showing a decrease of their efficiency and thus required the probe to be regularly (= every 6 months) changed.
With the Bioruptor, there is - to my knowledge - just nothing to be changed. But the machine must somewhat wear-out from the ultrasound waves, as "conventional" sonicators do.
I really interested to know whether your experienced a similar observation with your own machines.
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