Hi All,
Has anyone had any experience trying to add UMI to the smart-seq2 protocol? I've done some searching, but I haven't found an account...
5 results in 0.0027 seconds.
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UMI in Smart-seq2 protocol
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Variance in cDNA amplification
Hello,
I recently generated cDNA from 100 T-cells (from the same source) using the Smart-Seq2 protocol from Sandberg et al. After the pre-amplification... -
SmartSeq cDNA validation with TapeStation
Hi All,
I have RNA extractions from fungal fruiting body tissue, produced by cryo-sectioning-->LCM-->PicoPure RNA extraction kit.... -
BROAD cDNA-preparation - random primed or oligo-dT?
Hi,
I am looking into the RNA-Seq data from BROAD institute (SRA: SRX099331) and wonder whether the cDNA was created by random priming... -
the second strand cDNA synthesis in RNA-seq library generation
Hi everyone,
i am in the middle of RNA-seq now, and i wonder what are the ways to synthesize the second strand cDNA currently in RNA-seq library...
