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  • PrimerBuffalo
    Junior Member
    • Mar 2017
    • 6
    #1

    UMI in Smart-seq2 protocol

    Hi All,

    Has anyone had any experience trying to add UMI to the smart-seq2 protocol? I've done some searching, but I haven't found an account of anyone doing so. I would like to know if it is even possible.

    Thank you!
    Tags: cdna synthesis, rna-seq, smart-seq2, umi
  • nucacidhunter
    Jafar Jabbari
    • Jan 2013
    • 1250
    #2
    For this protocol UMI can be added to RT primer and SMART oligo. But they will be lost once amplified cDNA is fragmented/tagmented. With some modifications it is possible to keep primer and SMART oligo UMIs but reads from main body of transcript (except termini) will not have UMI.

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