27 results in 0.0025 seconds.
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Can I use secondary antibody as my negative IP control in my ChIP seq experiment?
I am doing a ChIP experiment for H3K9me3 marks in HEK293T cells. We have the ChIP grade Ab for H3K9me3 but didn't order ab for isotype control. We have... -
Chip Preparation Solution recipe?
Hello,
we have several Ion PI v2 chips, and we're successfully using them with Hi-Q chemistry kits. However, we're running short of Chip Preparation...Last edited by r.rosati; 04-18-2017, 09:57 AM. -
ChIP-seq - all QC looks fine, but low number of peaks with sequencing
Hi all,
I've been struggling with some ChIP-seq experiments for a while now, and I'm wondering if there's something obvious I'm missing.... -
Bioanalyzer for ChIPed DNA
Dear All,
I have a question regarding bioanalyzer for ChIPed DNA.
When I do bioanalyzer for the input DNA, I get peaks at the proper range...Last edited by zgene; 06-13-2016, 12:21 PM. -
error in Trimmomatic command
Hello,
I am running Trimmomatic command for ChIP-seq data
...Code:/home/bsharmi6/ChIPseqTools/Trimmomatic-0.36/trimmomatic-0.36.jar SE
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ChIP-chip to Coverage?
Hello to Everyone!
I am looking for a way to visualize data from ChIP-chip into Genome browser, and perhaps doing some aggregation plots... -
Genotypes conversion (SNP chip)
Hi all,
I have a big input file from 28 individuals which were genotyped by 600 K SNP chip (Affymetrix). I have about ~500,000 genotypes for each... -
Barcode Contamination - iChIP
Hi everyone,
I have attempted to perform indexing-first ChIP (iChIP) as described here: http://www.ncbi.nlm.nih.gov/pubmed/25103404. The... -
macs2 -- peak calling error on non-model genome
Hi there,
I'm attempting to use macs2 (v 2.0.10 20120605 (tag:alpha)) to call peaks from a ChIP-seq experiment on a non-model organism... -
Does 'verifying by Westernblot after Crosslinking really work?'
Hi all:
I am working on a in vivo Chip-Seq experiment. The protein I am interested in is a transcription factor. I made an N-terminal... -
Polymerase for ChIP-seq. Which do you use?
Dear all,
I would like to know which polymerase do you use for your ChIP-seq libraries?
We use the phusion polymerase, but... -
How to subtract a coverage track of mock IP from a set of ChIP tracks?
I have a set of ChIP data, most of them with a transcription factor, but also control data, which was not the raw input chromatin, but instead they performed... -
No DNA in ChIP IgG Control - Strange?
Hi Guys,
Thanks in advance for your help!
Background:
It's my first time doing ChIP. I am testing a native ChIP protocol... -
D. Melanogaster ChIP
I recently tried to do a ChIP-like experiment on chromatin from drosophila S2 cells and found that a large portion of my ChIP reads, but also my input... -
Using X-ChIP Kit for N-ChIP
I want to use the Invitrogen MAGnify ChIP kit to investigate histone binding of H3K4 & H3K27 but I do not want to cross-link my cells as:
...
