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Tools for cleaning PCR hood?
I have a really hard time reaching the back walls of my PCR hood to clean it. Does anyone know of any specific tools you can use to make it easier to... -
Cleaning amplicons before Nextera + Miseq run??
Hi Guys,
Quick question: I'm planning on sequencing large amplicons (5-15 kbp) on our MiSeq using the Nextera kit. Wondering the best... -
What Assemblers do, if there are Ns in the reads?
I'm dealing with Illumina data for transcriptome study.
I could not find the clues about what the assembers (e.g. Trinity, Oases, SOAPdenovo-trans,... -
96 well PCR clean up
Anyone found a 96 well clean up method that will remove 100 nt/bp and smaller from ~250 bp product? Most give 100 bp as the cutoff, but we've gotten... -
Covaris Maintainance
Hello All, the covaris S2 maintenance schedule says I should use 10% bleach for a monthly rinse, can I use a HazTab soultion or household bleach? or should... -
tagcleaner.pl 26% of my sequences match the tag NNNNNNNNNNNN
tagcleaner.pl is a wonderful tool, and it's ability to find unknown sequence tags is very useful.
However after cleaning out several tags...Last edited by john1923; 07-29-2011, 06:56 AM.
