Cleaning amplicons before Nextera + Miseq run??

[SDK]

Hi Guys,

Quick question: I'm planning on sequencing large amplicons (5-15 kbp) on our MiSeq using the Nextera kit. Wondering the best way to clean the PCR product before I run it through Nextera? Are there beads for cleaning large fragments? Any suggestions would be great.

Thanks!

[DRYTCYV]

I use Ampure beads for PCR cleanup.

https://www.beckmancoulter.com/wsrportal/wsrportal.portal?_nfpb=true&_windowLabel=UCM_RENDERER&_urlType=render&wlpUCM_RENDERER_path=%2Fwsr%2Fresearch-and-discovery%2Fproducts-and-services%2Fnucleic-acid-sample-preparation%2Fagencourt-ampure-xp-pcr-purification%2Findex.htm

[ScottC]

We use the same thing, but by Corning/Axygen... Axygen Axyprep.

[SDK]

Thanks guys. I thought beads would be the best choice but feared they may not work on really big amplicons. Great to know you guys have used them with success. Cheers!