Hello,
We received the raw reads of our ddRAD library from a sequencing facility, and after demultiplexing, I noticed that there is a 10-fold...
23 results in 0.0029 seconds.
-
ddRAD raw read count difference by barcode
-
Itru 5 and itru 7 stubs with ApeK1/BamH1 ddrad
Hi,
Sorry if this is in the wrong section. I am just wondering if the itru5 and 7 stubs would be compatible to barcode my samples when using both... -
Modified seq Illumina Primers for ddRAD
Hello, my ddRAD libraries (mean 550 pb) contain the first 5 identical bases at their ends, belonging to the partial restriction site. They will be paired-end... -
Mapping reads to find coding regions
Hi all,
I recently did a ddRAD study of a specific mussel species (which doesn't have a reference genome yet), and now I want to see if... -
Beads cleanup after enzyme digestion in RADseq: Yes or no?
Hi all,
I am working on double digest RAD sequencing, and I have quite a general question. In the ddRAD protocol of Peterson et al. they... -
ddRAD Post-PCR Bioanalyzer Has Multiple Peaks
Hello,
I have a problem with a post-PCR ddRAD library that was prepared using Peterson (2012). It was prepared along with another index... -
ddRADSeq Libraries
We seem to be having some difficulties creating reproducible ddRADSeq libraries.
We are noticing that after running the first of the processing... -
Is there a program dealing with degenerate base region (DBRs) (ddRAD seq protocol)?
I use ddRADseq with DBR to deal with PCR duplicates. We basically add a DBR, in the Read2 paired end.
My questions are:
- How can
-
Problematic ddRAD library post-size selection bioanalyzer trace
Hi everyone,
I'm preparing a ddRAD-seq plate for Illumina HiSeq 4000 sequencing and I'd like to know if anyone has seen similar Bioanalyzer... -
ddRAD, STACKS, and Trimming
Please disregard/delete.
Last edited by Carcharodon; 07-13-2017, 04:51 PM. -
[ddRAD] on removal of duplicate reads
Hi All,
Is it advisable to remove duplicate reads from double digest RAD (ddRAD) data generated on Illumina Hiseq platform ?
... -
Alternatives to Pippin Prep for ddRAD?
My lab is interested in doing population connectivity/phylogeography on herps, and think that ddRAD is probably the best NGS option for us (since we don't... -
ddRAD: Performing PCR BEFORE Size-Selection?
Greetings!
A quick search yielded a thread asking a similar question, but it went unanswered.
Up-front, my question is:... -
ddRAD/GBS lib conc on HiSeq 3000/4000 ?
Hi all,
Has anybody any advice on the "best" concentration (pM) to load a ddRAD or GBS libraries on a HiSeq 3000/4000 ? ddRAD... -
Is ddRAD sequencing strand-specific?
Hi all,
I have ddRAD libraries that I am trying to build an assembly-based reference, but I am not sure if ddRADseq sequencing reads are...
